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Tissue Culture

The CHIRI Tissue Culture facility houses four separate tissue culture rooms within its PC2 facilities. Each of the rooms is fully equipped to allow all aspects of cell culture including, but not limited to: incubators calibrated for temperature and gas composition, class II Biological Safety Cabinets, pipettes, S1 Pipette Fillers, centrifuges, water baths, fridges, freezers, microscopes and hemocytometers. We also provide cell storage facilities consisting of cryogenic storage dewars and ultra-low freezers.

Learn more about CHIRI’s Tissue Culture shared resource facility below.

General
Four TC Rooms
Mycoplasma Testing
Liquid Nitrogen Storage

General

Open hours

Appointments with CHIRI Tissue Culture facility staff are available 8AM–4PM Monday and Thursday and 10AM–1PM Tuesday and Wednesday (excluding Curtin-observed public holidays). Trained users can access the Tissue Culture Rooms 24/7.

Four TC Rooms

The Tissue Culture area is divided into four rooms based on the type of work performed.

TC1 is designed for primary human cultures not tested for any known human pathogens, viral work, parasite work and Mycoplasma status positive cell lines undergoing treatment.

TC2 is for cell lines with Mycoplasma status unknown (cell lines new to the facility), with only one Mycoplasma negative result and primary cultures negative for known human pathogens (most animal-based work from the SPF facility).

TC3 hosts cell lines with minimum two negative Mycoplasma results.

TC4 is designated for cell lines with minimum three negative Mycoplasma results cultured without antibiotics.

Mycoplasma Testing

Details

Mycoplasma species are the smallest and simplest self-replicating bacteria that are serious microbial contaminant of cell culture systems.

Mycoplasma contamination alters many eukaryotic host cell functions including growth, morphology, metabolism, genome and antigenicity. Mycoplasma can constitute up to 50% of the total protein and 15-30% of the isolated DNA and cause significant changes in micro array and gene expression profiles. Therefore using mycoplasma-contaminated cultures in experiments would result in the generation of erroneous experimental results.

Mycoplasma status of all cell lines is regularly checked in the facility using PCR method and all new cell lines introduced to the facility undergo quarantine.

Please see link to protocol ‘Sample Preparation for Mycoplasma Testing’.

Please contact CHIRI Tissue Culture Facility Staff to arrange testing of your cell lines (see ‘Contact us and feedback’ section).

Liquid Nitrogen Storage

Long term storage of all mammalian cell lines should be below -1320C. Approximately at this temperature water reaches its glass transition phase which means that all liquid water is completely frozen and all metabolic activity ceases ensuring long term sample viability.

The frozen vials of cell lines are distributed between four different dewars depending on where the cells were grown.

Please contact CHIRI Tissue Culture Facility Staff if you would require space for your cell lines in the dewars.

Training Facility training procedures

Training is a prerequisite to independent access to the CHIRI Tissue Culture shared resource equipment. Please contact Tissue Culture Facility Staff to arrange the training (see ‘Contact us and feedback’ section). The minimum training requirements are outlined below:

  1. Attend a General Tissue Culture Induction

All users must attend a General Tissue Culture Induction that involves two components: theory and practical.

Theory | The theory component covers basic principles of the Tissue Culture including aseptic technique, contamination, cells and cell growth. Theory also covers introduction to the TC facility equipment, Liquid Nitrogen storage, handling of the solid and liquid waste and booking system.

Practical | The practical component is laboratory based, and includes correct handling and standard operating procedures. Hands-on training with continues cell lines is provided and includes: cells seeding, media change, cells splitting and cells counting.

To book your induction and training sessions please contact CHIRI Tissue Culture Facility Staff.

Please also see links to the ‘Tissue Culture SOP’ and ‘Aseptic Technique’.

  1. Refresher training

If it has been more than two years since you started working in the Tissue Culture facility a refresher training on aseptic technique can be arranged. Please contact CHIRI Tissue Culture Facility Staff to arrange the training.

Contact us and feedback Who to contact about Tissue Culture

CHIRI’s Tissue Culture Facility Staff are always interested in ways to improve the equipment and services we offer to support your research endeavours. If you have an enquiry or want to provide feedback, please contact:

CHIRI Facilities Manager

Rob Steuart

Email | r.steuart@curtin.edu.au

Tel | +61 8 9266 7362

Mobile | 0419 192 971

CHIRI Senior Technical Officer (Tissue Culture)

Joanna Kelly

Email | Joanna.kelly@curtin.edu.au

Tel | +61 8 9266 1634

360° Tissue culture lab

Immerse yourself in a 360° virtual experience of our state-of-the-art tissue culture laboratory.