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Tissue culture

For sample processing the facility provides tissue culture and histology facilities. Tissue culture is used to grow cells by simulating conditions (in vitro within the lab) that may occur within the body. Cells can be cultured as an immortal cell line or directly from tissues or blood. Histological techniques provide a visual means for the examination and analysis of cell/tissue physiology. This can include embedding and sectioning tissues so that they can be visualised. Samples processed using tissue culture or histology are often analysed within the flow cytometry, metabolic analysis and microscopy facilities.

Tissue culture facility

There are 4 levels of tissue culture activity mainly based on Mycoplasma status. Mycoplasma is a bacteria which can significantly impact on cell growth and interpretation of biological results.

  1. Quarantine” work i.e. primary human cultures which have not been tested for any known human pathogens (most human work), viral work, parasite work and Mycoplasma status positive cell lines which are being treated.
  2. Dirty” cell lines which are Mycoplasma status unknown, only one Mycoplasma negative result or irregularly tested for Mycoplasma and primary cultures which are negative for known human pathogens (most animal-based work from the SPF facility).
  3. Clean” cell lines which have been regularly tested for Mycoplasma and have two negative results minimum.
  4. Ultra Clean” cell lines which have been regularly tested for Mycoplasma and have three negative results minimum and cultured without antibiotics.

We conduct regular induction sessions for tissue culture.

For further details please contact:

Joanna Kelly: joanna.kelly@chiri.edu.au

360° Tissue culture lab

Immerse yourself in a 360° virtual experience of our state-of-the-art tissue culture laboratory.